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rap1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rap1
    Rap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sc+47695/RAP1+Antibody/pmc12993239-119-51-53
    Average 94 stars, based on 530 article reviews
    rap1 - by Bioz Stars, 2026-09
    94/100 stars

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    other:

    Article Title: Activated GTPase-based assays and kits for the diagnosis of sepsis and other infections
    Article Snippet: Materials and Methods Antibodies Monoclonal rabbit anti-RAP1 (5G7): sc-47695 and rat monoclonal anti H-Ras (259): sc-35 were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, Calif.) Monoclonal mouse antibodies: anti-Rho (A, B.

    Article Title: Rapid parallel flow cytometry assays of active GTPases using effector beads
    Article Snippet: Antibodies Monoclonal rabbit anti-RAP1 (5G7): sc-47695 and rat monoclonal anti H-Ras (259): sc-35 were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA).

    Article Title: Rapid parallel flow cytometry assays of active GTPases using effector beads
    Article Snippet: Monoclonal rabbit anti-RAP1 (5G7): sc-47695 and rat monoclonal anti H-Ras (259): sc-35 were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA).

    Article Title: Activated GTPase-based assays and kits for the diagnosis of sepsis and other infections
    Article Snippet: Materials and Methods Antibodies Monoclonal rabbit anti-RAP1 (5G7): sc-47695 and rat monoclonal anti H-Ras (259): sc-35 were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, Calif.) Monoclonal mouse antibodies: anti-Rho (A, B, C) clone 55 (#05-7788), anti-Rac1, clone 23A8, #05-389, the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488, Rabbit Polyclonal anti Cdc42 #07-1466 were obtained from Millipore (Temecula, Calif.).

    Article Title: Activated GTPase-based assays and kits for the diagnosis of sepsis and other infections
    Article Snippet: Antibodies Monoclonal rabbit anti-RAP1 (5G7): sc-47695 and rat monoclonal anti H-Ras (259): sc-35 were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA) Monoclonal mouse antibodies: anti-Rho (A, B, C) clone 55 (#05-7788), anti-Rac1, clone 23A8, #05-389, the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488, Rabbit Polyclonal anti Cdc42 #07-1466 were obtained from Millipore (Temecula, CA).



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    EPAC1 SIM mutation interferes with cAMP - induced cellular activation of Rap small GTPases. A , schematic representation of the GST-RalGDS-mediated Rap-GTP pulldown. B and C , levels of cellular <t>Rap1-GTP</t> ( B ) or Rap2-GTP ( C ) in HEK293 cells ectopically expressing EPAC1-EYFP or EPAC1(SIM5A)-EYFP in response to 007-AM treatment. Data are presented as Mean ± SEM (N = 3). ∗∗ p < 0.01; ∗∗∗ p < 0.0005. EPAC1, exchange protein directly activated by cAMP; SIM, SUMO-interacting motif.
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    Image Search Results


    EPAC1 SIM mutation interferes with cAMP - induced cellular activation of Rap small GTPases. A , schematic representation of the GST-RalGDS-mediated Rap-GTP pulldown. B and C , levels of cellular Rap1-GTP ( B ) or Rap2-GTP ( C ) in HEK293 cells ectopically expressing EPAC1-EYFP or EPAC1(SIM5A)-EYFP in response to 007-AM treatment. Data are presented as Mean ± SEM (N = 3). ∗∗ p < 0.01; ∗∗∗ p < 0.0005. EPAC1, exchange protein directly activated by cAMP; SIM, SUMO-interacting motif.

    Journal: The Journal of Biological Chemistry

    Article Title: A SUMO-interacting motif in the guanine nucleotide exchange factor EPAC1 is required for subcellular targeting and function

    doi: 10.1016/j.jbc.2025.110279

    Figure Lengend Snippet: EPAC1 SIM mutation interferes with cAMP - induced cellular activation of Rap small GTPases. A , schematic representation of the GST-RalGDS-mediated Rap-GTP pulldown. B and C , levels of cellular Rap1-GTP ( B ) or Rap2-GTP ( C ) in HEK293 cells ectopically expressing EPAC1-EYFP or EPAC1(SIM5A)-EYFP in response to 007-AM treatment. Data are presented as Mean ± SEM (N = 3). ∗∗ p < 0.01; ∗∗∗ p < 0.0005. EPAC1, exchange protein directly activated by cAMP; SIM, SUMO-interacting motif.

    Article Snippet: Primary antibodies used in this study are anti-EPAC1 antibody 5D3 (Cell Signaling Technology, Catalog no. 4155, 1:3000 dilution), anti-RanBP2 antibody (Santa Cruz Biotechnology, Inc, Catalog no. sc-74518, 1:1000 dilution), anti-Rap1 antibody (Santa Cruz Biotechnology, Inc, Catalog no. sc-65, 1:1000 dilution), anti-Rap2 antibody (BD Biosciences, Catalog no. 610215, 1:2000 dilution), and anti-SUMO2/3 antibody (MBL Life Science, Catalog no. M114-3, 1:3000 dilution).

    Techniques: Mutagenesis, Activation Assay, Expressing